Well, at least the preliminary work. I'm trying to analyse G-protein coupled receptor heterodimerisation  via reconstitution of two (enhanced) yellow fluorescent protein fragments. This means that I'll have to construct plasmids for fusion proteins of the appropriate receptors with either the N- or C-terminal part of YFP. In close proximity due to receptor interaction, the two YFP halves should re-associate to form a functional fluorophore. Right now I'm busy with primer design and which restriction enzyme sites to implement. Sounds lovely, doesn't it?
 to be precise: neuronal nucleotide and purinergic receptors.
Labels: final year research