From cells to plasmid
Over the last days, one of my laboratory quests was carrying out a couple of maxi-prep plasmid DNA isolations via alkaline lysis and a Caesium Chloride ultracentrifugation. Today - eventually, after a dialysis and phenol-chloroform purification step - I bore the fruits of my hard work when I precipitated the relatively pure plasmid in ethanol. And merely for the matter of showing you the beauty of a «snow-storm in an eprouvette» I signed up at YouTube and uploaded a little movie-clip I made. You have permission to be amused by my Austrian accent.